895 Functional point mutations (Q730E) of ERAP1 revers HaCaT cells on proliferation, apoptosis and secretion by binding TNFR1 via inhibition of caspase-8 activity

We investigated the effect of functional point mutations (Q730E) endoplasmic reticulum aminopeptidase 1 (ERAP1) on proliferation, apoptosis and cytokine secretion HaCaT cells. The ERAP1 ERAP1-Q730E were overexpressed in expression TNFR1 TNFR2 cells was detected by immunofluorescence. proliferation CCK-8. Cell cycle analyzed flow cytometry. IL-1β, TNF-α IL-6 ELISA. IKKs caspase-8 Western blot. overexpression inhibited TNFR1, but not markedly increased decreased After overexpression, there significantly fewer number G0/G1 phase higher S M phase. levels TNF-α, IL-1β supernatants On contrary, cell promoted apoptosis, suppresses progression, levels. Functional mutation Q730E could reverse possibly binding inhibiting pathways.